Parkin Gene And Expression On Parkinson Disease

Objective:Study structural change and RNA transcriptional level of Parkin gene in patients with Parkinson disease and analyze the association between these changes and the etiology in the disease.Methods:Detect the Parkin gene DNA's exons and RNA transcriptional level of 15 Parkinson patients samples and 14 samples of control group by polymerase chain reaction(PCR),agarose gel electrophoresis,single strand conformation polymorphism(SSCP) and RT-polymerase chain reaction(RT-PCR).The deletion mutations and point mutations of the exons were identified by agarose gel electrophoresis and single strand conformation polymorphism(SSCP).And in the samples with abnormal SSCP result,further sequencing was performed to confirm the mutation and its location.The genetic expression were identified by agarose gel electrophoresis,gray scale value can be gained by measuring corresponding strap of objective fragment andβ-actin fragment,ratio of gray scale value should be analyzed.Results:We found no deletion mutations of the exons in all the patients and the controls.Expression of Parkin gene was absent just only in 1 Parkinson patient;There were no significant differences in Parkin gene expression between patients and the control group;however,Parkin gene expression of male patients was lower than female patients(P＜0.05).Conclusions:Our finding suggests that deletion mutations might not be a susceptible factor for part of PD patients.Expression of Parkin gene in Parkinson patient did not decrease obviously;however,Parkin gene expression of male patients was lower than female patients,maybe it related to male PD patients.