The Study On The Role Of Kit Positive Interstitial Cells In The Rat Bladder In Mechanisms Generating The Autonomous Bladder

Objects:to identify the presence and distribution of kit positive interstitial cells in rat bladder smooth muscle and characterize their morphology and ultrastnictural features;to study whether the kit positive interstitial cells played a role in the mechanisms generating spontaneous activity events;to evaluate the relation between kit receptor tyrosine kinase and the physiology function of kit positive interstitial cells and alterations of kit receptor tyrosine kinase at conditions of excessive mechanical overload.Materials and methods:Bladder obtained from the normal and mode of detrusor overactivity in rat were prepared for immunohistochemical investigations using the primary antibody of the kit receptor tyrosine kinase, and ultrastnictural studies using transmission electron microscope. Enzymatically dispersed cells were showed by scanning electron microsope. In this study,we had characterized the spontaneous activity of the urinary bladder smooth muscle from normal rat, the mode of detrusor overactivity induced partial outlet obstruction and stained by methylene blue,partically MB plus light using isometric muscle tension recording techniques.We also examined effets of Gleevec on spontaneous activity ,and accessed mRNA of kit receptor tyrosine kinase at the conditions of excessive mechanical overload through RT-PCR.