The Effect Of Promoting Apoptosis Of Human Cholangiocarcinoma Cells With Bcl-2 MRNA-cleaving Ribozyme

Cholangiocarcinoma is a fatal malignant tumor which origins from bile duct epithelial cells, and many therapies have no good effect on it at present. bcl-2 oncogene is a kind of anti-apoptosis gene, which and its protein can restrain multi-factor induced cell apoptosis and prolong cell life-span when over-expressed. Recent studies discovered that bcl-2 oncogene protein expressive male rate is high in cholangiocarcinoma cells, which maybe has close correlation with their cacoethic prognosis. We utilized synthetic gene of bcl-2 mRNA-cleaving ribozyme to cut down the level of bcl-2 expression in cholangiocarcinoma cells and its anti-apoptosis role, and induce the cells to apoptosis.RZ gene was cut from pGEM-3Zf (-) RZ by two enzymes first of all, the RZ gene segment was recovered by electrical elution-, after being cut by two enzymes, the carrier pDOR-neo and RZ gene were recombined under the T₄DNA ligase; recombined carrier was identified with enzyme cutting. The retrovirus carriers recombined, witch contains gene of bcl-2 mRNA cleaving RZ, were transfected into human cholangiocarcinoma cells QBC₉₃₉ by Lipofectamine intervened DNA transfection. Transfection and transcription of objective gene, bcl-2 express level and apoptosis of the human...