Effects Of Telomerase Antisense RNA And Bcl-2 Ribozyme On Hepatocarcinoma Cell Telomerase Activity Expression And Apoptosis

Deficiency of telomerase activity expression in normal cells can lead to theloss of telomere segments and cells' aging and apoptosis. Malignant tumors canget a chance of unlimited proliferation due to the activation of telomerase andthereby overcoming the shortening of telomere. Telomerase activation is regardedas the molecular basis of unlimited proliferation of malignant tumors. It is of greatsignificance to the pathogenesis of malignant tumors. Inhibition of telomeraseactivity can lead to the restoration of the shortening of tumor cell telomeresequence and the reactivation of the cells' aging pathway. Therefore, telomerasecan be taken as an ideal target in treatment of malignant tumors. Hepatocellularcarcinoma is one of the common malignant tumors, which is greatly harmful tohuman health. Based on the previous experiments conducted in our department, inthis study in situ hybridization technique was employed to detect the expressionof telomerase RNA in the primary hepatocarcinoma tissues. Then genetransfection technique, in combination with TRAP method, the flow cell device,TUNEL, cytobiology and EM, was used to observe the biology of the humantelomerase antisense RNA in hepatocarcinoma cells. Results showed that inhuman primary hepatocarcinoma tissues marked expression of human telomeraseRNA was detected while low expression or no expression occurred in cirrhosisand peri-carcinomatic tissues, and that there existed significant differencesbetween hepatocarcinoma tissues and non-hepatocarcinoma tissues (p<0.05).Transfection of human telomerase antisense RNA remarkably inhibitedSMMC7721 cell telomerase activity expression: TRAP-PCR-ELISA found thatSMMC7721 cells presented high-level telomerase activity, and absorbance(A₄₅₀-A₆₃₀) was 2.861, whereas cells (SMMC7721/pBBS212-hTR) receiving...