Gene Cloning And Expressions Of Human Antibody Fab Fragment And DsFv Against HBsAg

Over the last century animal antiserum, and more recently rodent monoclonal antibodies, have been used clinically to neutralize toxins, and to treat bacterial and viral infection. However, the use of animal antibody can lead to an antiglobulin response and hypersensitivity reactions. Human engineering antibody is the third generation of antibody, which overcomes the shortcoming of antigenicity among species in antiserum and monoclonal antibody. It has a potential therapeutic use in human diseases. Great efforts have been made on this area by a lot of laboratories and medical companies in the word. Some of engineering antibodies have obtained the approval of FDA. Many others entered clinical trials. The objective of this research is to explore and pursue the techniques about high level expression of the human antibody of Fab fragment and dsFv against HBsAg.Human immunoglobulin combinatorial library was generated by using phage surface display expression system, from which phage antibodies(Fab fragments) against HBsAg were screened, and their antigen binding activity and specificity were assayed by ELISA and competition inhibition ELISA. A human phage antibody against HBsAg was obtained. Its genes were sequenced by 373A automatic DNA sequence analysis machine. The results showed its heavy chain gene was whole, but its light chain gene was lost. The V_H gene of the selected...