The Relationship Study Between ANP Gene And Essential Hypertension In Xinjiang Kazakhs

Objective: To investigate the relationship between ANP gene T2238C, G1837A single nucleotide polymorphism (SNP), gene haplotype and essential hypertension in Xinjiang Kazakhs. Methods: 543 Xinjiang Kazakhs were recruited in our study. They were divided into 314 hypertensives (EH group) whose SBP>140mmHg and/or DBP> 90mmHg and 229 normotensives (NT group) whose BP<140/90mmHg. We measured their ANP gene T2238C, G1837A polymorphism by.PCR-RFLP method and tested the association of the polymorphism and heplotype with hypertension. Results: 1) We detected three kinds of genotype and two kinds of allele of ANP gene T2238C polymorphism. The frequencies of three kinds of genotype TT, TC, CC were 0.87, 0.11, 0.02 in hypertensive patients and 0.91, 0.08, 0.01 in controls respectively. The frequencies of two kinds of allele T and C were 0.93, 0.07 in hypertensives and 0.95, 0.05 in controls respectively. We also detected three kinds of genotype and two kinds of allele of G1837A polymorphism. The frequencies of genotype GG, GA and AA were 0.79, 0.20, 0.01 in hypertensives and 0.83, 0.16, 0.01 in controls respectively. The frequencies of G and A allele were 0.89, 0.11 in hypertensives and 0.91, 0.09 in controls respectively. 2) Both of genotype and allele frequencies of T2238C and G1837A had no significant differences between two groups (x~2 =2.013, P=0.36 and x~2 =1.022, P=0.312; x~2 =2.249, P=0.13 and x~2 =0.583, P=0.445 respectively). 3) We did not find association of 2238C allele with...