The Structure And Function Of Two Cancer-related Genes, The Effect Of Isoforms Of The Cell Polarity Protein, Human ASIP, On The Cell Cycle And Fas/FasL Mediated Apoptosis In Human Hepatoma Cells And The Transformation Role Of A New Gene, F-lana

Here, we reported the structure and function of human cell polarity protein, ASIP, and a new gene, F-lana. We firstly investigated the effect of the two isoforms of cell polarity protein, hASIP, on the cell growth and Fas/FasL mediated apoptosis in BEL-7404 human hepatoma cells. We also proved the transformation role of F-LANa in NIH3T3 cells for the first time.(i) Our group previously found that there were, at least, five isoforms of human cell polarity protein gene, asip. Interestingly, the exon17b of hasip encoded the aPKC binding site of hASIP protein in which the presumed phosphorylation site was included. The isoforms included two long forms, hASIP-la (exon17b containing long isoform) and hASIP-lb (exon17b deleted long isoform), two short forms, hASIP-sa (exon17b containing short isoform) and hASIP-sb (exon17b deleted short isoform), and hPAR3. We also found that the variants containing exon17b were expressed in both normal liver tissue and human hepatocellular carcinoma (HCC), and the expression of exon-17b-deleted variants was downregulated in HCC. More work in our lab reported here showed that hASIP-sa might accelerate cell growth through the modification of S phase, while hASIP-sb could not. Growth inhibition by hASIP-a...