Expression Of The Polarity Protein ASPP2,Par3 And Occludin In Psoriatic Epidermis

Objective:Psoriasis commonly is known as "psoriasis" is a kind of epidermal hyperplasia and dermal inflammation characterized by common skin disease,bring tremendous physical and mental suffering to the patient,long-term treatment also brings heavy economic burden and drug side effects to the society and the individual.However,the mechanism of the formation of its skin damage has not yet been made clear.Histopathologically,it is mainly the hypertrophy of the spinous layer,the shortening of the mitosis cycle of the keratinocytes and the shortening of the epidermis through the time of the epidermis;Microscopically,the intercellular connection between the lesions of the psoriasis area is loose and the normal intercellular close connection is lost.The formation of intercellular close connections is accompanied by the gradual formation of cell polarity.It is considered that the change of epidermal polarity is an early event in the pathogenesis of psoriasis vulgaris,which is associated with the disorder of cell polarity.This study mainly uses immunohistochemical,RT-PCR and Western-blot methods,detection of polarity proteins ASPP2,Par3,Occludin in psoriasis,psoriatic skin lesions(eczema,lichen planus and nodular prurigo),skin tumors(SK,SCC and BCC)expression and distribution,discuss the possible role of ASPP2,Par3 and Occludin in the formation of psoriasis,providing a new theoretical basis for the further diagnosis and treatment of psoriasis.Methods:Collection of specimens in 129 cases:normal 10 cases(it was taken from theoutpatient department of our hospital for cosmetic or plastic surgery for 2015-2017 years,and there were no other related skin diseases);the psoriasis group 20 cases,psoriatic skin lesions in 55 cases(15 cases of eczema group,LP group of 20 cases,20 cases of nodular prurigo),skin tumor in 44 cases(16 cases of group SK,group SCC 13 cases,BCC group of 15 cases),the specimens were taken from the outpatient and hospitalized patients in our hospital for 2015-2017 years.Neither system nor local treatment had been accepted.The expression and distribution of ASPP2,Par3 and Occludin in psoriasis,psoriatic skin lesions and skin tumors were detected by immunohistochemistry,the levels of ASPP2,Par3,Occludin mRNA and protein expression in psoriasis area were detected by RT-PCR and Western-blot.Results:ASPP2 is mainly expressed in the nucleus of psoriasis,psoriatic skin lesions,skin tumors and normal skin.Compared with the immunohistochemical staining intensity(++)of ASPP2 expressionin in normal skin,the expression of ASPP2 by immunohistochemical staining intensity is(+ + +)in psoriasis,psoriatic skin lesions,skin tumor.Is distributed in the whole layer of the epidermis(mainly in the basal layer and the spinous layer in the normal skin),the expression of mRNA and protein in psoriasis was significantly higher than that in normal skin(P<0.001);Par3 is mainly expressed in the cytoplasm of psoriasis,psoriatic skin lesions,skin tumors(SK,BCC)and normal skin(mainly expressed in cell membrane in SCC).Compared with the immunohistochemical staining intensity(++)of Par3 expressionin in normal skin,immunohistochemical staining for Par3 expression intensity in psoriasis,psoriatic skin lesions and skin tumors were(+),(+ +),(+ + +).All of them are distributed in the whole layer of the epidermis(normal mainly on the basal layer),the expression of mRNA and protein in psoriasis was significantly lower than that in normal skin(P<0.001);Occludin is mainly expressed in the cytoplasm of psoriasis,psoriasis like skin lesions,skin tumors and normal skin.Compared with the immunohistochemical staining intensity(+)of Occludin expressionin in normal skin(mainly concentrated in the spinous layer and the granular layer),noobvious positive cells were found in the whole layer of psoriasis,psoriasis and skin tumors,the expression of mRNA and protein in psoriasis was significantly lower than that in normal skin(P<0.001).Conclusion:The expression and distribution of polar proteins ASPP2,Par3 and Occludin in psoriasis,psoriatic skin lesions and skin tumor are abnormal,indicating that the dyspolarity of epidermal cells is one of the pathogenesis of psoriasis,psoriatic skin lesions and skin tumors;ASPP2,Par3 may be involved in the formation of psoriatic lesions through genetic and immune mechanisms.P53,STAT and cytokines are involved,STAT1 can act as an upstream regulator of ASPP2,regulate ASPP2 transcription,ASPP2 promotes MET,and form psoriasis like lesions;In skin tumors(SK,SCC,BCC),distribution and dyeing enhancement of ASPP2,it is contrary to the tumor conclusion of other tissues,indicating that the regulatory pathway of ASPP2 in skin tumors may be completely different from other tissue tumors.Par3 plays a carcinogenic role in skin tumors,and the incidence of SCC may be associated with Par3 positioning error.