The Treatment Of Phlegm-DAMP Type Amenorrhea And Empirical Study On IR Of Ovaries

Polycystic ovary syndrome is a common female internal secretion disease. It not only appears as an endocrine disease and also is a reproductive barrier. It is found out that polycystic ovary syndrome is closely related to insulin resistance. However, all existing researches concentrate to insulin function target organization, including skeletal muscles, fat and liver except ovary. It is inaccurate to assume that ovary function barrier is due to low insulin blood sickness barrier. Moreover, ovary junction barrier only exists in multi-pouches ovary syndrome patient, but not common in Type 2 diabetic patients. The glycometabolism of polycystic ovary syndrome patient is exceptionally related to insulin resistance and ovary function barrier. Ovary is the fundamental function unit of female reproduction. It involves insulin and signal protein and metabolism enzymes. Therefore this research has been established in vitro ovary insulin resistance model. It expresses the gonadotrophic hormone signal conduction and the insulin signal conduction in reciprocity. In vivo, it is a long-term chronic pathology process. With tritium sign examination cell and insulin resistance, the research stresses on gonadotrophic Hormone signed conduction reciprocity.On the foundation, troglitazone is taken as the positive control medicine. With the use of Chinese herbal medicine, insulin is more sensitive. In the research, dexamethasone is used in pig ovary granular cells. Together with tritium, the cell absorbs less glucose.Thus insulin resistance is established. With medicines-troglitazone, metformin, berberin, and diadzein, more glucose is absorbed. This improves the insulin resistance.Ovary granular cell excretes estradiol and progesterone. RT-PCR and Western Blot technology are used to discuss insulin signal transmission way, relating to glucose absorption. The main theme is on insulin resistance and ovary function. The Findings are:1.Using dexamethasone, level of insulin glucose transportation proteins Glut-4 mRNA remarkedly drops. Use of troglitazone, metformin, berberin and daidziein, Glut-4 mRNA level remains high. Absorption of glucose improves.2.When ovary granular cell has insulin resistance, IRS-A m RNA reduces. Use of Troglitazone, metformin, berberin and diadzein improves the IRS-1 mRNA.3.The peroxide enzyme body multiplication activation acceptor increases when there is insulin resistance. Use of troglitazone, metformin, berberin and daidzein reduces it.4.Akt, also known as protein activating enzyme B(PKB) is the serine protein activating enzyme. Akt is a PI-3K circuit essential member which relates to glucose synthesis and transportation. When there is insulin resistance, Akt M RNA drops. This does not affect after use of troglitzone, metformin, berberin and daidzein. 5.PI-3K is one kind of lipid activating enzyme of insulin metabolism. P1-3K P85 and PI-3KP110 are main ones. In insulin resistance, the protein expression drops. Use of troglitazone, metformin, berberin and daidzein improves it.6.P450 arom protein is closely related to a gene. This is an important aromatized enzyme transforms and catalyzes androgen and estrin. In insulin resistance, the P450arom protein and the P450arom gene are very high. Use of troglitazone, metformin, berberin and daidzein reduces them.7.After use of dexamethasone for a long time, the insulin growth factor ILGF-IR of overy drops. It is improved with use of troglitazone, metformin, berberin and daidzein. In Conclusion:In dexamethasone , it reduces PPAR y protein. It also reduces Glut4mRNA and the protein expression level hindering glucose transportation protein production. It lowers the level of insular signal transmission main protein IRS-1. Where there is insulin resistance, P450 arom mRNA rises. Thus estrin level elevated. This causes reproduction barrier. The use of troglitazone, metform, berberin and daidzein improves all these. Lastly, troglitazone is withdrawn clinically because of high liver toxicity. It is only used in vitro as its effect is very good. The remaining Chinese herbal medicines are useful too, namely, Daiazein extract of gegen, Huang qin from berberin and salvia miltiorrhiza alkone from saliva miltiorrhiza.