Experimental Research Of Effect Of Fibrin Glue Associated With Antisense On Vein Graft Restenosis

Part I Experimental Research of Effect of Fibrin Glue as ExternalSupport on Preventing Vein Graft RestenosisObjective. Mechanical methods to reduce vein graft disease have been postulated. And use of a bioabsorbable, nonrestrictive external support around vein graft has been proven to have beneficial effects on reducing neointimal and medial thickness. However, it is limited by potential complications. To explore a novel alternative external support, in the present study we investigated the effect of fibrin glue on preventing vein graft failure as perivenous application.Methods. 24 New Zealand male rabbits were divided into non-supported group (NS group) (n=12) and fibrin glue group (FS group) (n=12). All animals underwent unilateral jugular vein into common carotid artery interposition grafting and then fibrin glue was applied as perivenous support. Samples of tissues were harvested after 4 weeks and histological and immunohistochemical analysis were underwent. Results. The vein grafts with perivenous support by fibrin glue demonstrated a statistically decrease in neointimal and medial hyperplasia compared with non-supported vein grafts (p<0. 01) . Immunohistochemical analysis showed that the expression of PCNA was inhibited significantly in FS group compared with NS group. Almost intact endothelium was showed in FS grafts (p<0. 01) . But in NS grafts there were severe endothelium damages where lymphocytes infiltrated. In NS grafts, the smooth muscle cells were hyperplasia and migrated to intimal, whereas in FS group, the hyperplasia of smooth muscle cells were inhibited and migrated to adventia. We also observed the FS grafts were associated with a profound network of matured microvessels, but in NS grafts they were far less numerous.Conclusions. Perivenous application of fibrin glue has beneficial effects on reducing neointimal and medial hyperplasia of vein grafts. These effects were related to intact endothelium, inhibition of PCNA expression, adventitial migration of SMC and formation of numerous microvessels.