Correlation Between VEGF And Angiogenesis In Lung Carcinoma, And Antiangiogenic Therapy Based-on VEGF And Its Receptors

Lung carcinoma is one of the leading cause of cancer death, and the formation of new blood vessels (angiogenesis) is essential for the growth and metastasis of tumor. Anti-angiogenesis therapy has become an important strategy for antitumor. Vascular endothelial growth factor (VEGF) is a critical cytokine and major factor implicated in angiogenesis. VEGF induce the proliferation of endothelial cells (ECs) and the formation of micro vessel, promote the permeability of microvessels via its receptors, Flt1 and KDR. The angiogenesis may be Inhibited by antagonizing VEGF-mediated signaling.Objective The aim of the present study was to estimate the correlation between VEGF and its receptors (Flt1, KDR) and angiogenesis in lung carcinoma. Based on angiogenesis , we systemically studied the PH â…¡ -7, a new antitumor agent, in vitro and in vivo, in order to explore its mechanism and search for a new anti-agniogenic drug.Materials and Methods (1) MVD, VEGF, Fltl, and KDR was detected by immunohistochemistry in 49 resections of tumor. (2) VEGF and its receptors mRNA were investigated using in situ hybridization in resections of 16 patients. (3)In vitro, PH â…¡ -7 anti-tumor activity was determined using MTT. With or without this agent, the VEGF and its...