Comparative Studies On The Biologic Effects Of Retinoic Acid And RATS₁ Gene On Human Esophageal Cancer Cells In Vitro

Retinoic acid (RA) has long been considered as a potent differentiation-induc agent. It plays an important role in the regulation of cell proliferation ε differentiation as well as in embryogenesis of vertabrates. In squam(?) differentiating tissues, RA may control squamous cell differentiation. RA is a one of the most potent agents in differentiation inducing and proliferat(?) suppressing activities of human tumor cells. A wide variety of tumor cells s(?) growth and begin differentiation by RA treatment. Mass chemoprevention show that RA-derivative retinamid can suppress or block the malignant transformat(?) of hyperplastic esophageal mucosa in high risk area of esophageal cancer China. We have previously shown that all-trans RA could inhibit growth a induce differentiation in human esophageal cancer cells in vitro. In 1990, constructed two cDNA libraries from a human esophageal cancer cell 1 EC8712 before and after RA-treatment. Through repeated subtract: hybridization of these two cDNA libraries and appropriate screening procedun cDNA sequence designated RATS1 was isolated and characterized. In pres study we treated two human esophageal cancer cell lines, EC109 and EC87 with all-trans RA and RATS1 in order to compare their biological effects and elucidate the underlying molecular mechanism.