The Experimental Research And Clinical Observation Of Free Fat Graft Autotransplantation

The wide and varied applicaton of free fat graft autotransplantation in surgery illustrates that free fat autografts have provided a useful material for soft-tissue replacement and augmentation, but the results obtained so far are quite unpredictable, with wide variations in the resulting bulk of the grafts. Disappointed by this situation some surgeons were obliged to anticipate the possible use of cultured preadipocyte suspension as an injectable soft-tissue augmentation material.To explore the feasibility of above idea and for the later need of in vitro study on adipocyte behaviour, we cultured the adult human adipocyte and preadipocyte with initial success. The culture midium we used was a mixture of DMEM / F12 medium ( 1:1; v / v ) containing 20% newborn calf serum, 15 mM NaHCO₃,15mM HEPES,33μM biotin.17 μ M pantothenate. Insulin, dexamethasone.and bFGF were added after the cells adhered to the bottom of culture dishes.We observed the cellular "islets" in the adult human preadipocyte cultures, as demonstrated by Carraro (1990) when culturing rat mature adipocyte. The core of the islet was undigested stromal-vascular debris, around which arranged the actively proliferating preadipocytes.These cells grew quickly into local monolayer conflueuce and differentiated at a high percentage. The scattered preadipocytes revealed slow division rate and could also differentiate independent of confluence. This behaviour difference dueing to their relations with stromal debris indicated the regulatory influences of stromal components on preadipocyte. In cultures with few or no mature adipocyte, the preadipocyte multiplied but rarely differentiated.Thus the expected...