The Effects Of Exogenous P16 Gene On Radiosensitivity Of Human Nasopharyngeal Carcinom Cell Line CNE-2

p16 gene is an important tumor suppressor gene, and has high frequency of alteration in both primary nasopharyngeal carcinoma (NPC) and NPC cell lines, but the role of p16 gene in NPC has not been studied further, so we study the role of p16 gene in NPC. CNE-2 cells of NPC cell lines were chosen and exogenous p16 gene was transfected into CNE-2 cells by lipofectin. After G418 selection, we obtained p16 stablly expressed NPC cell clones of CNE-2-p16. Compared with primary CNE-2 and CNE-2-vect cell lines, the studies and results were presented as follows: 1). Exogenous p16 gene was transfected into CNE-2 cells by lipofectin and express p16 protein which was confirmed by immunohistochemical detection. That indicated exogenous p16 gene had been transfected into CNE-2 cells successfully and expressed actively. 2). Growth of CNE-2 cells was inhibited significantly by p16 gene. 3). Assay of flow cytometry to CNE-2-p16 cells showed arrest in G1 phase, decrease in percentage of S phase cell significantly . 4). Conlony formation of CNE-2-p16 cells in soft agar showed significant...