Experimental Studies Of EGFR Gene Suppression By RNA Interference On Enhancing Radiosensitivity Of Ovarian Cancer Cells

Objective: To trans- fect that had constructed expression vector of EGFR specific siRNA into human ovarian cancer cell line SKOV3, then evaluate the effect of RNA interference (RNAi)on the target gene expression and radiosensitivity of the cell line.Methods: EGFR sh- RNA was synthesized in vitro and transfected into SKOV3 cells with lipofectamine technique and then the positive clones were selected by G418. The positive RNA interference cell line,negative RNA interference cell 1ine and the normal control cell 1ine were included in the study.RT-PCR and Western Blot technique were performed to detect the inhibitive effect of RNAi on EGFR mRNA level and protein level,respectively.Three kind of cell lines were accepted different dose of radiation.Cell survival curve was drafted by MTT method.Results:The expression of EGFR gene was surpressed obviously in the specific sequence shRNA group,and EGFR mRNA and protein surpression rates were 77.5% and 76.1%,respectively ;Sequence-specific shRNA-EGFR can enhance significantly the radiosensitivity of ovarian cancer cells,and significant statistic differences were existed among three groups(P<0.05).Conclusion: The experiments in vitro confirmed that EGFR gene silencing by RNAi can enhance significantly the radiosensitivity of ovarian cancer SKOV3 cells.