The Cellular Mechanism Of Human Parathyroid Hormone (1-34) Treatment Effect On Osteoporosis

Because the percentage of menopause and postmenopause women have been increasing along with the longevity of the mankind, osteoporosis has become a worldwide problem affecting the old's health , and its incidence rate has increased gradually.The main cause of osteoporosis is the imbalance between bone resorption and bone formation due to many reasons, i. e. bone resorption >bone formation. So the key question to the treatment of osteoporosis is to modulate bone remodeling successfully. There are a number of reports about intermittent infusion hPTH(1-34) increasing bone formation while decreasing bone formation continuously infusion. This suggest5 that hPTH(1-34) may be useful for the treatment of osteoporosis by an appropriate administration. But the mechanism remains obscure. Osteoblasts is the only cell line in bone that containing PTH receptor, the target cell line of PTH in bone. The main function of osteoblasts is to increase bone formation. In this paper, we try to invest the process of hPTH(1-34) on osteoblasts in order to classify the cellular mechanism of hPTH(1-34) treatment on osteoporosis. The experiment is divided into two parts.Part I: The effect of human parathyroid hormone(1-34) on osteoblastic cell cultures isolated from newborn rat calvaria1. Osteoblasts were isolated from newborn rat calvaria by sequential...