Isolation Of Binding Proteins For Advanced Oxidation Protein Products From The Surface Of Human Monocytes

Advanced oxidation protein products (AOPP) are dityrosine-containing protein cross-linking products isolated from the plasma of hemodialysis (HD) patients, which was firstly reported by Witko-Sarsat in 1996. AOPP represent a generic name for final products of oxidative modification of proteins caused by oxidative stress in HD patients. AOPP rise as novel uremic toxins with the progression of chronic renal failure (CRF) and oxidative stress and accumulate in patients'plasma and tissues. It has been clarified that AOPP are a novel class of pro-inflammatory mediators with monocytes being their elective cellular target. AOPP are able to trigger the oxidative burst of monocytes and their release of inflammatory cytokines, and join the process of systemic micro-inflammation in CRF patients, which is a crucial pathogenic link to long-term uremic complications, such as dysregulation of immune system, accelerated atherosclerosis and dialysis-related amyloid arthropathy. However, the formation of AOPP is nonreversible and their combination with proteins in vivo greatly hinders the removal through hemodialysis, which results in the limitation of therapy. The future development in therapy for AOPP should aim at their acting pathways and the search for new interference targets to block their toxic effects, on the basis of which proper medications can be screened and new ways of blood purification can be designed. Therefore, the study of of AOPP is important and necessary.Till now, the acting mechanism of AOPP remains elusive. Study shows that AOPP bear numerous similarities and significant relationship with advanced glycation end products (AGE) in formation, structure and biological activities. AGE are the end products of nonenzymatic glycoxidation of proteins and also inflammatory mediators. They act via a series of binding proteins present mainly on the surface of monocytes, among which receptor for advanced glycation end products (RAGE) is the best knewn one. Taking into account the significant resemblance between AOPP and AGE, the involvement of a similar...