The Underlying Neural Mechanisms Of Peripheral Snake Venom And Bee Venom Induced Hyperalgeisa

In clinic, pathological pain is one of the most frequently observed clinical symptoms, which is characterized by a persistent spontaneous pain, hyperalgesia and touch-evoked pain (allodynia). In order to elucidate the underlying mechanisms of the development and chronicity of pathological pain, it is necessary for us to develop suitable animal models which can mimic the process of clinical pathological pain. During the past several decades, many animal pain models were established such as the formalin test model, the CFA model, the partial sciatic nerve ligation model (PSL) model and the chronic constriction injury of the sciatic nerve model (CCI). Though these pathological pain models mentioned aboved provided us powerful tools in the field of pain study. These models still have some pitfalls in the study of pathological pain since they are maybe fit for the study of certain kinds of pain behaviors. Till now, more animal pain models were newly set up to explore the neural mechanism of pathological pain.1. In the current project, morphological methods and behavioral observation were adopted to elucidate the underlying mechanism of the snake venom induced pathological pain. The results are as followings:(1) We observed that marked demyelination occurred in the ipsilateral sciatic nerve samples taken 2 w after snake venom injection into the sciatic nerve. There was little changes in myelin sheath of the sciatic nerves samples taken from NS injection into the sciatic nerve.(2) By immunohistochemistry, it was observed that SP, CGRP, GAL and VGluT1...