| Impairment of learning and memory would more and more be a worldwide issue as aged people account for a large population in both developing and developed countries, and it would cause the discomforts and problems in physical, psychiatric and social aspects. Nevertheless, this terrible declination also somewhat affects some groups of young and middle aged people. Hence, the research of antiageing drugs becomes a widely concerned topic.Securinine is an alkaloid isolated from Securinega suffruticosa. Some pharmacological studies convinced that securinine had a stimulative effect on central nervous system (CNS), especially on the spinal cord. A radio- ligand binding test confirmed that securinine had a specific binding ability on γ-amino butyric acid (GABAa) receptor, so it was supposed that its stimulation on CNS was related to its antagonistic effect against GABA. The present study was to investigate the effect of (+) securinine on improving learning and memory deficits and facilating synaptic transmission, and its mechanism.Part one. In vitro protective effect of (+) securinine on cell toxicity induced by AP(25-35) and GABA in PC12 cells and primary cortex neurons.1. In vitro protective effect of (+) securinine on cell toxicity of AP(25-35) in PC12 cellsPC12 cells, incubation with aged Ap(25-35) for 48 hours, were markedly toxicant and reduced by a death rate of 80% (MTT detection). (+) Securinine could alleviate the cell toxicity induced by AP(25-35) in a dose dependent manner, as 10-7 mol/l securinine has an obvious effect.2. In vitro protective effect of (+) securinine on cell toxicity of GABA in primary cortex neurons... |
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