Changes Of Subsets Of Th Cells And Dentritic Cells I In The Bone Marrow Of Severe Aplastic Anemia Patients

Purpose:1.To detect the quantity, ratio of Th1 cells and Th2 cells in bone marrow of severe aplastic anemia (SAA) patients at active and recovery phases;2. To measure the levels of IL-4, TNF-α in serum of patients with SAAat active and recovery phase;3. To evaluate the relationship between the cytokine of Thl type(TNF-a), effective cell of type Thl (CD₃⁺CD₈⁺ cell), the cytokine of Th2 type (IL-4) and reticulocytes (Ret), absolute number of neutrophils (ANC) of patients with SAA; the relationship between the numbers of Thl cells and TNF-α or CD₃⁺CD₈⁺ cells in bone marrow of patients with SAA; and explore the role of Thl cells and balance of Th1/Th2 in the pathogenic mechanism of SAA;4. To measure the percentage of CD_1a⁺CD_11c⁺, CD₈₃⁺CD_11c⁺ cells in bonemarrow of patients with SAA at active and recovery phases and normal controls, and evaluate the relationship between the numbers of CD₈₃⁺CD_11c⁺ cells and Thl cells or CD₃⁺CD₈⁺ cell, to explore the role of CD₈₃⁺CD_11c⁺ cells in the pathogenic mechanism in SAA. Methods:...