Empirical Study Of Cotransfecting Antisense Oligonucleotide For Treating Human Pancreatic Cancer And Observing With MRI Dynamically

This study adopted antisense oligonucleotide targeting K-ras and antisense oligonucleotide targeting the insulin-like growth factor-1 receptor to carry out the therapy experiment for pancreatic cancer in vitro and in vivo.The effect of therapeutic alliance of these two antisense oligonucleotides was investigated.This study adopted large scale and high field MRI ,and adhibited microscopy coil first time for developmental observation of mouse bearing cancer.The imaging appearance of mouse bearing cancer was studied thoroughly.Part One : Point mutation and its type of K-ras of human pancreatic cancer cells Patu8988 dectected using PCP-SSPObjective: To dectect the point mutation and its type of K-ras oncogene codon 12 of human pancreatic cancer cells Patu8988 using PCR-SSP. Materials and Methods: Genome DNA of Patu8988 cells of exponential phase of growth was extracted. With genome DNA as template, three upstream primers (R1,R2,R3) targeting point mutation of K-ras oncogene codon 12 and common downstream primer(R4) were added respectively. PCR was employed to amplify corresponding fragment with Taq DNA polymerase.After 12% native polyacrylamide gel electrophoresis, ethidium bromide staining, observed and took photographs with ultra-violet transmission analysator. Results: Bright electrophoresis strip appeared at R2-R4(GTT mutation) matched-pairs. The molecular weight of amplified...