| ObjectiveTo investigate the changes of angiotensinâ…¡(Angâ…¡), its receptors (AT1R) and NGF on the diabetic rat model with erectile dysfunction (ED), then using angiotensin receptor blocker (ARB) and exogenous NGF to treat the diabetic rats with ED, so as to evaluate if there is any relationship between them and pathogenesis of diabetic ED.MethodsMale Sprague-Dawley rats were injected with 65 mg/kg streptozotocin to induce DM. The diabetic rats with ED were selected by hypodermic injection of apomorphine after 8 weeks of model setting. The levels of Ang II of blood and penis were determinated by radioimmunoassay, while the distribution of AT1R in penile tissue was detected by staining. The level of NGF was determinated by using RT-PCR and Western Blot. The intragastric administration of valsartan was kept on for 8 weeks, compared to the treatment of spironolactone and NS. The exogenous NGF was also used to treat the diabetic ED rats, compared to insulin. The APO test and ICP determination were used to evaluate the erectile function of each group.ResultsCompared with the control group, Angâ…¡levels of blood and penile tissue in DM group ,as well as the level of blood in DED group, were very high and differences were significant statistically(P<0.05). Angâ…¡of tissue in DED increased sharply (P <0.001). The AT1R changed oppositely against the level of Angâ…¡. The erection frequency in Vilsartan group was higher, compared with other two groups (P<0.05), in which no significant difference was observed (P >0.05). There was no significant difference in erection rate among these three groups. ICP in Vilsartan group was higher, compared with other two groups (P <0.05), in which no significant difference was observed (P >0.05). Compared with that of...
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