Ultrasound-Target Microbubble Destruction Induces Exogenous Gene Expression In Cell Lines And Murine Organs

ObjectiveThe aims of this study were to determine whether ultrasound target microbubbles destruction can be used as a method to enhance the expression of the report or therapic genes in vitro and in vivo with high efficiency and tissue specificity. With further refinement, it may represent an effective nonviral avenue of gene therapy for genetic diseases.MethodTransgene expressed in ECV304 cell line and the heart of the mouseAir filled microbubbles (2.0-4.0μm in diameter) were created by sonicating the mixture of human albumin, glucose, mannitol and special additive designed for stabilization. Plasmids DNA pEGFPN₁...