Experimental Study On Antitumoral Efficiency Of IFN-γ Gene Transfection By Ultrasound Mediated SonoVue Microbubble Destruction In Vitro And In Vivo

To study the efficiency and feasibility of targeting gene transfer to tumor cells or tissues by ultrasound mediated microbubbles destruction in vitro and in vivo.We transferred the plasmid pcDNA3/IFN- Y into human hepatic cancer cell line SMMC-7721 in vitro by ultrasound mediated SonoVue microbubbles destrction(UMMD). Cell viability was assayed by Trypan Blue staining, and IFN-γ expression in their supernatants were monitored by ELISA, the percentage of the apoptotic cells were measured with flow cytometric analysis .The results demonstrated that microbubbles and ultrasound radiation did not effect on cell viability.The significant enhanced expression of IFN- γ was found in the cells treated with UMMD,with an increased percentage of the apoptosis cells.SMMC-7721 cells were inculated subcutaneously in nude mice, two weeks later,mice were allocated randomly into 5 groups: intratumoral injection of PcDNA3/IFN-y only (p); plasmid injection combined ultrasound radiation (U+p); intratumoral injection of PcDNA3/IFN-γ combined microbubbles (m+p) ;microbubbles and plasmid injection with...