The Experiment Research On The Role Of CD8～+T Lymphocyte In Respiratory Syncytial Virus (RSV)-induced Asthma

Part â… Comparison of the difference in sensitivity among Hep-2, Vero and HeLa cells to respiratory syncytial virus (RSV)Objective: To compare the difference in sensitivity among Hep-2, Vero and HeLa cells to RSV infection. Methods: Hep-2, Vero and HeLa cells were infected by RSV respectively, the period and the character of cytopathic effect (CPE) were observed. The expression of RSV antigen was evaluated by direct immunofluorescence assays (DFA). The levels of RSV mRNA in different cells were tested by reverse transcriptase polymerase chain reaction (RT-PCR). Results: The CPE in HeLa, Hep-2 cells occurred in the fifth day after RSV infection, and the CPE in Vero cell occured in the seventh day. HeLa cells became swelling and round, but not form syncytium, which was obviously observed in Hep-2 and Vero cells. It is also observed that the expression of RSV in Hep-2 cells was the greatest by RT-PCR and DFA. Conclusion: The sensitivity of the three kinds of cells to RSV infection is different, and the sensitivity of Hep-2 cells to RSV infection is the greatest among of them, and whose CPE have completely different character in morph.