Experimental And Clinical Study Of Kansui Root And LMWH Combining Therapy For Severe Acute Pancreatitis

Background After SAP developing, the proinflammatory cytokines storm lead to Microcirculation disturbance and Systemic inflammatory response syndrome (SIRS) or Multiple organ failure( MOF). Recent researches have found that nuclear factor - kappaB(NF- κ B) and inflammatory mediators, Thromboxan2(TXA2)/Prostacyclin I2(PGI2) play important role in the development and progress of SAP.So decreasing excessive release of inflammatory mediator and improving the microcirculation of pancrease are two important ways in treatment of SAP.Our previous experimental studies had confirmed that treatment of LMWH and kansui root could improve the microcirculation disturbance and inhibit the inflammatory reaction of SAP in rats.Our ward researches have showed that kansui root had functions of immunosuppression, anti-inflammatory , improving the microcirculation of gastrointestinal tract,etc. The purpose of this present study is to investigate if there is a synergism of LMWH and kansui root in the combining treatment of SAP in rats.Methods One hundred and fifty SD rats were randomly divided into 5 groups; (l)Sham group(A group):30 rats;(2)Severe acute pancreatitis group (B group):30 rats; (3)therapeutic group with kansui root(C group):30 rats;(4): therapeutic group with LMWH(D group):30 rats;(5) combining therapeutic group with kansui root and LMWH(E group);30 rats. The blood samples and tissue samples of each group were gathered at 2,6,12,24 hour after the operation respectively for following test:â‘ serum amylase level;â‘¡serum tumor factor-1 α (TNF-α ) and interleukin-6(IL-6)level; â‘¢ thromboxane-B2(TXB2) and 6-keto-prostagdin-F1 α (6-keto-PGF1 α ) level in pancreatic tissue and plasma; â‘£expressions of Cyclooxygenase-2(COX-2) and NF-κ B/P⁶⁵ protein in pancreatic tissue; ⑤changes of pancreas, lung, liver, kidney tissue...