Association Of Essential Hypertension And Heart Period Signal Spectra With Nitric Oxide Synthase Gene Polymorphism Determined By Allelic Specific Primer Polymerase Chain Reaction Techniques

Objectives(1 )To study and establish an allelic specific primer polymerase chain reaction (ASP-PCR)technique system and to apply this technique to study single nucleotide polymorphism(SNP) of genes.(2) To study genetype combination distribution and allelic frequency distribution of SNP at 5 sites of nitric oxide synthase (NOS) gene including NOS3 T-786C, NOS3A-922G, NOS3 G894T, NOS2 C-1173T and NOS2 G-954C SNPs.(3 ) To study genetype combination distribution and allele frequency distribution of SNPs of inducible nitric oxide synthase(NOS2) of NOS2 C-1173T and NOS2 G-954C associated with essential hypertension in the Chinese Han nationality population.(4) To study allele genotype combination distribution and SNPs of NOS3 A-922G, NOS3 T-786C and NOS3 G894T associated with essential hypertension in the Chinese Han nationality population.(5 )To study the relationship between resting heart rate and SNPs at 5 sites of NOS gene including NOS3 T-786C, NOS3A-922G, NOS3 G894T, NOS2 C-1173T and NOS2 G-954C SNPs(6) To search for association between heart period signal spectrum features and SNPs at five sites of NOS2 G-954C, NOS2 C-1173T, NOS3 T-786C, NOS3 A-922G and NOS3 G894T in the Chinese Han nationality population.