Relationship Between Genetic Polymorphisms Of Endothelial Nitric Oxide Synthase Gene And Essential Hypertension

1、Studies on resin purification process optimization of eucommia ulmoides oliverand its antihypertensive effect mechanismBackground: Through research it is found that the barks, Oliver, stems, fruits andflowers of Eucommia ulmoides contain various compositions, such as lignin,cycloalkane and phenylpropanoids. The modern pharmacological research shows thatEucommia ulmoides has many functions, such as anti-aging, antitumor and bone cellproliferation. This paper studies the resin purification process optimization conditionsand antihypertensive effect of Eucommia ulmoides Oliver. Materials and Methods:The orthogonal experiment method is adopted to determine the purification effect ofvarious macroporous resin for eucommia ulmoides Oliver, and the SHR model is usedto determine antihypertensive level of eucommia ulmoides Oliver. Results: Theoptimal process of resin extraction and purification of Eucommia ulmoides Oliver isthat the loading liquid concentration is6mg/ml,20%ethanol is used as the eluent, theflow rate control is3.0column volume per hour and the diameter-height ratio ofadsorption column is1:6; the extracts of Eucommia ulmoides Oliver can reduce SHRblood pressure, its antihypertensive effect is relatively stable and has no significantimpact on HR, but the antihypertensive effect is lower than that of control medicinecaptopril. At the same time, the extracts of eucommia ulmoides Oliver cansignificantly increase the content of serum NO and reduce the content of ET.Conclusion: the Eucommia ulmoides Oliver can relax blood vessels, reduce theperipheral resistance, reduce the returned blood volume, and eventually achieve theantihypertensive effect. 2、Studies on the levels of plasma endothelin and serum nitric oxide inhypertensive patientsObjective：To investigate the levels of plasma endothelin, serum nitric oxide andNO/ET in hypertensive patients. To find the relationship between the levels of plasmaendothelin, serum nitric oxide and NO/ET with the extent of Essential Hypertensionand circadian rhythm of blood pressure in hypertensive patients. To find on the levelsof plasma endothelin, serum nitric oxide and NO/ET in hypertensive patients aftertreated by Duzhong pingya tablet and Lotensin. Methods：Levels of plasmaendothelin, serum nitric oxide and NO/ET were detected in hypertensive patients (n=232) and normal controls (n=130). In hypertensive patients there were89patientsof low risk,78patients of risk and65patients of high risk. Ambulatory blood pressuremonitoring for24h were done with hypertensive patients.74hypertensive patientswere treated by lotensin and69patients were treated by Duzhong pingya tablet fortwo weeks. Plasma endothelin, serum nitric oxide and NO/ET were detected after twoweeks’ treating in hypertensive patients. Ambulatory blood pressure monitoring for24h were done again at the same time. Results：In hypertensive patients the level ofplasma endothelin is higher than that of normal controls. While the level of serumnitric oxide is lower and NO/ET is much lower than that of normal controls. Asignificant difference was seen in the levels of plasma endothelin, serum nitric oxideand NO/ET between the two groups. The changes of these three levels were muchmore significant when the risk of hypertension was increasing and the changing ofblood pressure rhythm from dipper blood pressure group (n=109) to non-dipper bloodpressure group (n=123). To the levels of SBP, DBP, plasma endothelin, serum nitricoxide and NO/ET Lotensin (n=66) had significant effects after two weeks’ treating inhypertensive patients but no effects were seen in the group (n=70) treated by Duzhongpingya tablet. Conclusion：The levels of plasma endothelin, serum nitric oxide andNO/ET have something to do with not only morbility of Essential Hypertension butalso the extent of Essential Hypertension and the changing of blood pressure rhythm.To the levels of SBP, DBP, plasma endothelin, serum nitric oxide and NO/ET Lotensinhas more significant effects on Essential Hypertension than that of Duzhong pingyatablet after weeks’treating. 3、Relationship between genetic polymorphism of endothelial nitric oxidesynthase gene and essential hypertensionObjective：To detect the relationship between genetic polymorphism of endothelialnitric oxide synthase （eNOS）gene and essential hypertension．Methods：Polymorphism of the promoter region-786thsite T/C and894thsite G/T of eNOSgene were detected by polymerase chain reaction-restriction fragment lengthpolymorphism in hypertensive patients (n=215) and normal controls (n=108)．Inhypertensive patients there were82patients of low risk,72of risk and61of high risk.At the same time hypertensive patients were divided into dipper blood pressure group (n=102) and non-dipper blood pressure group (n=113) by the change of bloodpressure rhythm. OR and95%CI were studied to find the interaction betweenpolymorphisms of both two sides which we studied．Results： To eNOS promoterregion-786，the frequencies of TT, TC and CC genotype were22.79％,50.70％and26.51％in hypertensive patients and36.11％,52.78％and11.11％in normalcontrols．A significant difference was seen in the distribution frequency in the twogroups of hypertensive patients and normal controls．To the894thsite of the seventhextron of eNOS the frequencies of GG, GT and TT genotype were69.30％,22.33％and8.37％in hypertensive patients and82.41％,15.74％and1.85％in normalcontrols．Asignificant difference was also seen in the two groups.To promoter region-786and the894th site of the seventh extron of eNOS no significant difference wereseen when the risk of hypertension was increasing and the blood pressure rhythm waschanging from dipper to non-dipper blood pressure. When the genotypes of the twosites were CC and TT or TC and TT the OR of morbility of essential hypertensionwere much higher than that when they are TT and GG．Conclusion：Polymorphismsof the promoter region-786T/C and894thsite G/T of eNOS gene have something todo with morbility of essential hypertension but have nothing to do with the extent andthe change of blood pressure rhythm of essential hypertension．Genotypes TT of thepromoter region-786and GG of894thsite of eNOS gene may have some protectiveeffect against essential hypertension．There may exist some interaction betweenpolymorphisms of the two sites we studied．...