The Study Of Molecular Mechanism Of Curcumin On Inhibiting The Proliferation/Inducing Apoptosis Of Glioma Cell

By using glioma cell lines C6 from rats, and U87MG as well as LN229 from humen, the molecular mechanism of Curcumin in the proliferative inhibition and apoptotic induction of glioma cell, were studied. PTEN is another important tumor-suppressor gene that was discovered in the past a few years following P53, and the mutant rate of PTEN in glioma is around 40%. At present, there is no report on the wild type PTEN upregulated by Curcumin to inhibit the proliferation or/and to induce the apoptosis in glioma cell.Methods (1) MTT was used to measure the living cells treated with Curcumin; BrdU antibody labeled with fluorescence was used to observe the proliferation of C6 cells treated with Curcumin under the fluorescence-microscope; (2) PI was used to determine the cell cycle arrested by Curcumin with Flowcytometry; (3) Annexin V-PE and 7-AAD were used to measure the apoptotic cell at the early phase induced by Curcumin with Flowcytometry; Agarose electrophoresis of DNA fragmentation was used to determine the apoptotic cells at the late phase; (4) The fluorescence probe of DCFDA was used to measure the iROS production from C6 cell induced by Curcumin; (5)Real time RT-PCR was used to measure the mRNA expression from C6 cell induced by Curcumin. Western blot was used to determine the level of protein from C6 cell induced by Curcumin; (6)Gel-shift assay system was used to measure...