Experimental Study On Inhibitory Effects To RSV Infection By Combination Treatment Of Short Hairpin RNA And Deoxyribozyme

Respiratory syncytial virus (RSV) is the most common cause of acute upper and lower respiratory tract infections in infants, young children, and adults worldwide. There is no reliable vaccine or antiviral drug against RSV at present. RNA interference(RNAi) and Doxyribozyme(DNAzyme,DZ) technology are two potent methods to degrade expression of the aimed gene. They have been extensively applied in fields such as study of gene functions, tumor and virus infection. Alone with the developing of molecular biology and molecular virology, especially making great progress to the study of viral gene structure and function, viral replication inhibited on gene level especially on post-transcription has been becoming the hot spot at present. RSV M2 gene plays a important role in RSV transcription and replicatioan. In order to inhibit the replication of RSV on gene level, the recombinant plasmids targeting the mRNA of RSV M2 gene coding sequence capable of expressing short hairpin RNA(shRNA) were design and constructed. And the inhibitory effects of the specific shRNA recombinant plasmid(pshRNA) and DNAzyme on RSV replication in cell culture system were observed in this study.Partâ… Selection of Specific anti-RSV Short Hairpin RNA Target Sites and Construction and Identification of Short Hairpin RNA Recombination Plasmid...