Study On Anti-uterine Cervix Cancer Activity And Its Action Mechanism Of Gleditsia Sinensis Sting

Uterine cervix cancer is one of malignant tumors of genital system threatening women health and life, which may be resulted from socioeconomic factor, pathological changes of cervix, virus infection and reduction of immune function, and so on. The prevention and cure are difficult because of various pathogenesis. The therapy of uterine cervix cancer mostly uses chemosynthesis drugs at present, but side effects and multidrug resistance of chemosynthesis drugs become frequent in treatment. Naturally occurring drugs have been paid close attention by researcher for their low side-effect and no-drug-resistance. So screening naturally occurring drugs of anti-uterine cervix cancer becomes the study hotspot of pharmacology field.Gleditsia sinensis sting distributes widespread in China. It is adjuvant of anticancer in common use used by herbalist doctor. The study on pharmacological activity of Gleditsia sinensis sting had not been developed widely yet. No study on anti-cancer activity of Gleditsia sinensis sting and its effective constituent was reported.In our study, we investigated anti-uterine cervix cancer activity and its action mechanism of Gleditsia sinensis sting by animal model of transplanted tumor and Hela cells. The results were as follows.(1) The ethanolic extract from Gleditsia sinensis sting was prepared by hot refluence method. The effects of ethanolic extract on uterine cervix cancer harboring mice were studied. The results showed that it inhibited the solid tumor growth of solid tumor harboring mice and prolonged the survive of ascites tumor harboring mice. The tumor inhibition rate and the life prolong rate were 47.5%, 59.5%, 63.9% and 52.2%, 67.3%, 78.8% in low, middle and high ethanolic extract groups (250, 500 and 1000 mg/kg body weight). It showed that the ethanolic extract from Gleditsia sinensis sting had anti-uterine cervix cancer action in vivo significantly.(2) With the methods of plant chemical constituent subentry test, we found that Gleditsia sinensis sting mainly consisted of saccharide, saponin, flavonoids, hydroxybenzene, protein, polypeptide, organic acid, lignin, steride, triterpene, essential oil and alkaloid, and didn't contain tannic acid, iridoid glycoside, quinone and terpene lactone.(3) The flavonoids was extracted and refined by the methods of extraction step by step...