The Study About The Relationship Between The Formation Of Cholesterolcalculus And The Transports Of Liver Cell

ObjectiveCholesterol cholelithiasis is an exceptionally common and economically significant digestive disease. Epidemiologic and family studies indicate that cholesterol cholesterol calculus formation is in part genetically determined. Cholesterol cholesterol calculus disease represents a failure of biliary cholesterol homeostasis in which the physicalchemical balance of cholesterol solubility in bile is disturbed. The primary pathophysiologic defect in both mice and humans is unphys-iologic cholesterol supersaturation of gallbladder bile. Bile' s formation and excretion is to be thought correlated with transport protein of hepatocyte. The function of some of these transport protein had been clear - cutted, such as NTCP, BSEP, MRP2 ,MDR3 and SCP2. At present, these proteins function in cholesterol calculus' s formation had been proved in animal experiment. But the relationship between these proteins and cholesterol calculus' s formation in human is still short of evidence. Our investigation will study the relationship between these proteins and cholesterol calculus 's formation.MethodsTissue specimens 20 liver tissue specimens were taken from patients with cholesterol calculus , which were the trial group . 10 liver tissue specimens were taken from patients without cholesterol calculus, 5 were patient with hepatorrhe-xis, 5 were from department of remove , which were the control group .Methods All the collections were detected with RT - PCR, and 8 of these specimens were detected with Western blot. Statistical analysis was taken by SPSS 10.0.