Correlation Analysis Between Being Rich In Adiponectin And Gallbladder Cholesterol Calculus As Well As Abnormal Blood Lipids In C57BL/6J Mice

Objective:To observe the influence of injecting exogenous adiponectin on gallbladder cholesterol gallstone caused by high-fat diet in C57BL/6J mice; To observe the effect of injecting exogenous adiponectin on risk factors inducing gallbladder cholesterol calculus in C57BL/6J mice.Methods:40 C57BL/6J mice which randomly divided into experimental group(EG) and control group(CG) formed cholecystolithiasis induced by lithogenic diet (containing 15% cocoa butter fat,1.25% cholesterol, and 0.5% cholic acid), we created high adiponectin animal model by injection of Adiponectin Mouse Recombinant through vena caudalis and peritoneal cavity (0.1μg a mice·d, dissolved in 0.1ml deionized water), while CG were injected physiological saline(0.1ml a mice-d) in the same way for eight weeks. In the end of this experiment, plasma concentrations of total cholesterol(TC), triglyceride(TG), adiponectin(APN), apoproteinAl(apo-Al), apoproteinE (apo-E) and bile density of TC, bile acid, phospholipids were detected by the method of enzyme-linked immunospecific assay(Elisa);After anatomy of mice, we recorded the number having gallbladder cholesterol calculus; Paraffin-embedded gallbladder sections were stained with hematoxylin and eosin for morphology. For immunofluorescence investigation of CD68, fluorescent staining was used.Results:The prevalence rate of gallstones was significantly higher in CG mice (94.4%) than in EG mice (55.6%) (P<0.05) after 8-week feeding of high fat diet. Plasma TC, TG, apo-E were significantly higher in CG mice than in EG mice (P<0.05), but plasma adiponectin and apoA1 had adverse results (P<0.05). The biliary concentrations of TC was significantly different between CG and EG mice, the CSI was lower in EG mice than CG mice (P<0.05). The mean CD68 levels in gallbladder tissue were higher in CG.Conclusion:Being rich in adiponectin reduced the incidence rate of gallstones and modulated the level of TC in bile, as well as reduced CSI, influenced the risk factors caused by cholesterol calculus.