Molecular Mechanism Of The Vascular Oxidative Stress Induced By High Level Of Free Fatty Acids(FFAs) And Interfering With Traditional Chinese Medicine

Part one:Antioxidative Therapy Improving Insulin Resistance Caused by Elevated FFAs ConcentrationObjective: Elevated circulating FFAs concentration, a characteristic of metabolic syndrome, may induce systemic oxidative stress. Here we report that increased oxidative stress caused by elevated blood FFAs concentration is an important pathogenic mechanism of insulin resistance and antioxidative treatment can improve insulin sensitivity via restoration of normal reactive oxygen (ROS) production.Materials and Methods: Male S-D rats, 6~8weeks in age, were assigned to three groups and all underwent 6 hr iv infusions in each group: Controls (C, n=6): saline; FFA (n=6 ): 20% intralipidl.8uL/min+heparin 0.72IU/min; Intervention (I, n=6): GSH (1.2mg/kg/min ) and lipid/heparin. Serum FFAs level and GSH/GSSG ratio both in blood and erythrocyte were measured at baseline and after infusion. The euglycemic hyperinsulinemia clamp study was conducted at the last 2 hours of infusion to evaluate the peripheral insulin...