～(99m)Tc Labeled Anti-hepatoma McAb HAb18 Fragment For RII And The Action Of βG Prodrug To β-Glucuronidase-cDNA Transfected Hepatoma Cell Line HHCC

Anti-hepatoma McAb and the β-glucuronidase cDNA will provide useful tools to study hepatoma targeted diagnosis and gene therapy. In this paper, a serial experiments , ^99mTc labeled McAb HAb18 for radioimmunoimaging (RII) and β-glucuronidase cDNA used for hepatoma "suicide gene" ,were done . The whole studies were summarized as follows:1 ^99mTc labeled anti-hepatoma McAb HAb18 fragment for RII in nude mice bearing xenografted human hepatoma . Firstly, the anti-hepatoma McAb HAb18 was digested by active papain at different pH condition. At pH5.5, 37℃ for 18h, F(ab')₂ fragment was obtained; and Fab fragment was at pH8.0 37℃ for 1h , the production was purified by DEAE-FPLC。 The fragment purity was more than 90%. The yield was 40% to 60%. Secondly, In order to increase the sulfhydryol group , McAb Fragments were modified with 2-iminotholane and labeled with ^99mTc by transchelation from ^99mTc-GH .Finally, the ^99mTc-IT-F(ab')₂ and ^99mTc-IT-Fab were injected into the nude mice for RII. Results showed that both ^99mTc-...