Construction Of Antisense Eukaryotic Expression Vector Of Gene BC047440 And Its Exploration Of The Effect On The Growth Of Hepatocellular Carcinoma

Background:Human primary hepatocellular carcinoma (HCC) is one of the highly prevalent malignant diseases worldwide which carries a very poor prognosis, and the 5-year survival rate is limited to 5%.HCC, like other solid tumors, occurs in the context of various genetic and epigenetic changed and grows more and more malignant with the increase of altered molecules. Although a number of cellular genes have been described as involved during the HCC carcinogenesis, little is reported unique for HCC. Therefore, it is urgent for us to seek more new HCC-associated genes and make clear their structures and functions. Identification of novel HCC-associated genes will greatly improve our understanding of this disease at molecule level and will encourage us to develop new targets for gene diagnosis and gene therapy.At previous study, through the separation and idendification of differential expression gene between HCC tissues and the adjacent liver tissues we had found a novel cDNA fragment of HCC-associated gene by Suppression subtractive hybridization (SSH). Last year, we reported the identification and characterization of the novel human HCC-associated gene,which was BC047440. This gene was initially identified as a candidate gene that is overexpressed in HCC tissues (HCCs) but undetectable in nontumorous or normal liver tissues as determined by a differential display experiment. It was verified in vivo that gene is expressed preferentially in HCCs but not in the adjacent liver tissues at mRNA levels. In this paper,we reported the function of gene BC047440 in the growth of hepatocellular carcinoma .