Experimental Study Of Apoptosis And P53 Gene Therapy For Lung Cancer

Malignant neoplasms are difficult problems far from being under control. Molecular analysis shows that they are caused by alterations of oncogenes, antioncogenes and disorder of apoptosis. Up to now the etiology and pathogenesis have still not been understood. For that, to gain a further insight of malignancy, we had established a model of apoptosis induced by doxorubicin in a lung adenocarcinoma cell line A549 and transferred human wild-type p53 gene(wt-p53), a tumor suppressor gene, to A549 cells in vitro using retroviral vector to evaluate the theraputic effect of p53. The results were1. After treatment of 20uM doxorubicin for 4 hours and post-incubation for another 10 hours apoptosis was markedly induced in A549 cells with a series of characteristic signs including memberane buding, cytoplasmic condensation and esophilia, chromatin condensation, fragmentation, margination and apoptotic body formation. Agrose gel electrophoresis analysis revealed DNA cleavage (called UNA ladder)and there was also anapoptotic peak when analyzed with cytometry. All those results suggested that apoptosis of A549 cells...