The Role Of Lipopolysaccharides From Oral Anaerobic Bacteria On Human Pulpal And Periodontal Fibroblast And Bone Resorption In Vitro And Endotoxin Content In Periapical Granuloma

Lipopolysaccharides(LPS) from anaerobic bacteria in infected root cannals were found to have many biological activities.The ability of LPS to stimulate macrophages releasing TNF,IL-1,activate complement,act as a thymus—independent antigen and B lymphocyte mitogen, stimulate bone resonption and be cytotoxicity in cultures, however, does provide a possible key role for LPS in the pathogenesis of progressive periapical disease and other oral inflammatory discases. But, the mode of penetration of LPS into pulpal and periapical tissue and its interaction with host cells is not clear at present.The present studies were undertaken to evaluate the role of LPS in human pulpal and periapical region tissues in vitro. Our work include extracted LPS from Bacteroides and Fusobacterium involved in root cannal infection,studied the cytotoxicity of LPS on human pulpal fibroblast and...