Selection And Identification Of Mimic Epitopes For Gastric Cancer Associated Antigen

Filamentous bacteriophages have been used extensively in recent years for the display on their surface of large repertoires of peptides generated by cloning random oligonucleotides at the end of the genes coding for the phage coat proteins. The key attributes of phage display system are: the efficacy of biopanning, the ability for amplification and the linkage of the displayed peptide with its encoding DNA sequence within the phage particle, enabling quick and simple elucidation of binding sequences. Numerous papers have shown not only that this approach is successful with an extraordinarily wide range of protein targets, including structural proteins, signal transduction proteins, receptors, serum proteins, oncoproteins, viral gene products and enzymes, but also that it is possible to select displayed peptides that mimic the original characteristic of antigen binding to specific antibodies, without previous knowledge of the antigen structure. In this respect, phage technology is now well established as an important experimental approach in the development of novel vaccines and drugs.Aiming at the identification of possible immunogenic mimotopes for gastric cancer associated antigen, we used two monoclonal antibodies...