Production And Detection Of Monoclonal Anti-Idiotype Antibodies Against Vibrio Anguillarum

Vibrio anguillarum is the pathogenic bacteria of Vibriosis, which is an infectious disease found in various fish species. It can also infect people by food chain. Mouse hybridoma clones that produced monoclonal anti-idiotype antibodies to V. anguillarum ATCC19019 were established. There are a series of experiments including:1. To produce and characterize of monoclonal antibodies against V. anguillarum by fusing of Sp2/0 myeloma cells and spleen cells of Balb/c mice. Positive reaction was confirmed by enzyme linked immuno-sorbent assay (ELISA) with goat anti-mouse IgG-HRP. Eight hybridoma cell lines were obtained and were named as 2H2, 3B2, 3D2, 3A3, 3C4, 4A6, 4E6 and 4E12 . Identification of subgroup showed that 2H2, 3D2, 3B2 and 4E12 belonged to IgG₃; 4E3 belonged to IgG_2a; while 3A3, 4A6 and 3C4 were IgG₁. The titersof these mAb ascites were 1×10^-5-1×10^-7. Bacteria cross-reactionshowed that two 2H2 and 3A3 mAbs did not react with other vibrioes and they can be used to detect V. anguillarum. Other six mAbs can...