Experimental Study On Replacement Technology Of Aflatoxin B₁ Standard On Immunoassay

Aflatoxin is one of poisonous metabolizable substances of Aspergillus parasiticus and A. flavus that pollute foodstuff, its products and feeds.The major and the most poisonous is aflatoxin B₁(AFB₁) which has strong acute and chronic poison and is considered as I degree carcinogen by IARC.Because AFB₁ has many ways to pollute foods, it is impossible to stop pollution and the best method is to strengthen assay and control.But AFB₁ standard of higher concentration is had to be contacted during assay,and many personnels have jealous attitude about this strong carcinogen.Besides, as biological terrors occur frequently,many countries in the world retrain export and import of poisonous substance.So AFB₁ standard hampers assay for food safety seriously.According to the immunity net brought forward by Jerne,the body will cause a series of complicated immunity chain responses based on external antigen. Stimulated by antigen, the body generates Ab₁, Ab₁ generates Ab₂, then Ab₃, et al.So these make up of the comprehensive idiotype and anti-idiotype net.Antibody idiotype is the antigen determinant of antibody.Idiotype antibody(Ab₁) is the antibody with idiotype,and anti-idiotype antibody(Ab₂) is the antibody which is generated by antigen determinant of idiotype antibody. The different antigen determinant of Abi can generate different kinds of Ab₂ which is classified 4 types,that is, Ab₂a, Ab₂β,Ab₂γ and Ab₂e. Only Ab₂β has the effect of antigen internal image.It can imitate antigen to induce the generation of differential antibody against initial antigen. Ab₂β can replace AFB₁ in immunoassay of foods to carry out nontoxic assay and break out the limit of...