Establishment Of Animal Model For Hyertrophic Scar And Its Relative Study

The prevention and treatment of hypertrophic scar is the major researching content of tissue repair. It is the key to the solution to further know of pathological and molecular biological basis of the genesis and development of hypertrophictic scar. Traditionally, it is accepted that hypertrophic scar is unique to human and do not exist in animal. Due to the lack of the animal model of hypertrophic scar produced by animal itself, it is limited to study the occurrence, development and outcome of hypertrophic scar systematically.In order to explore the possibility of establishing an animal model for hypertrophic scar, we designed this experiment. Whole-layer skin defect on ventral and dorsal side of rabbit ear, whole-layer skin and cartilage were prepared with 46 ears of 23 rabbits, and TGF-β1 and IFN-γ are injected into the local regions after the heal of partial wounds. The result shows that it is more likely to form excessive dermal scarring on the ventral side of rabbit ear than that on the dorsal side, and it is not affected to form excessive dermal scarring after removing cartilage simultaneously, and local injection of TGF-β1 and IFN-γ can respectively promote or inhibit the formation of excessive dermal scarring, which is similar to the reaction of human hypertrophic scar. And then 216 6mm round wound on ventral side of 18 rabbits were divided into whole-layer skin group, saline injecting group, TGF injecting group, IFN injecting group and whole-layer skin with cartilage group. Within 30-100 days after wounding, we observed them comparatively at 6 time points and calculated their occurrence rates. The samples stained with HE, VG, and Masson were examined histologically, and the raising height at the wound sites were calculated under light microscope, and the results were analyzed statistically. With histochemical (Alcian Blue, AB) and immunalhistochemical (Streptoantibiotin-Paroxide, 2 step) methods, we studied the contents of proteoglycans and fibronectin in excessive dermal scarring of each group. The results show that wounds after healing, whether there is cartilage or not, can all produce excessive dermal...