| Aim: Proliferative vitreoretinopathy (PVR) is an excessively wound healing response that involved many kinds of inflammatory cytokines, and migration and proliferation of cells. Macrophages have been proved a role in the development PVR. Monocyte Chemotactic Protein-1 (MCP-1) can specifically recruit macrophages and lymphocytes into the inflammatory area. Retinal pigment epithelial cells (RPE) are predominant proliferative cells in PVR. This experiment aim to (1) examine the effects of cultured macrophage conditioned media (MCM) on RPE production and gene expression of MCP-1, (2) investigate the effects of MCP-1 on modulation of the migration and proliferation of RPE. Additional experiments were conducted to test the changes of this modulation effect under the action of several agents, and (3) investigate the relationship of the presence of MCP-1, macrophages and T-lymphocytes in specimens obtained from eyes with retinal detachment and PVR.Methods: (1) Human RPE were cultured with 20% macrophage-conditioned mediua (MCM), IL-1β (0.2ng to 20ng/ml) or TNF-α (0.2ng to 20ng/ml),and with additional dexamethasone (10-8, 10-7, 10-6M) or daunorubicin (2μg,20μg,200μg/L) for 2, 4, 8, 24 or 48 h. Secreted MCP-1 in the supernatant of cultured RPE was measured with Western blot assay, and intracellular MCP-1 was detected with in situ hybridization and immunohistochemistry. (2) We used an in vitro wound healing model in which a small area of a confluent monolayer of RPE was denuded with a razor blade. The cultures were subsequently incubated with MCP-1 (0.2ng/ml to 20ng/ml),...
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