| Objective: Prolifertive vitreoretinopathy (PVR) after ocular injury and retinal detachment (RD) is a series of cellular events that include migration, adhesion, and proliferation of dedifferentiated cell, followed by membrane formation and contraction along surfaces of the detached retina resulting in tragic fate. During this excessive process of wound healing, the retinal pigment epithelium (RPE) is one of the most importantly proliferating cells This study consists of two parts: To observe the proliferative state of migrated RPE in specimen obtained from vitrectomy in ocular injured patients and that of RPE in situ in experimental RD model. Method: 0. The proliferation of migrated RPE was detected by immunohistochemical tcchnique with monoclonal antibodies of Ki- 67 and proliferative-cell-nuclear-antigen (PCNA) for 22 injured eyes 4 The relationship between the prolifertive state of migrated RPE and injuried course, traumatic classification, complications which include RD and PVR, and the site where the specimen was taken from were studied. ? 18 eyes of cat were subdivided into continued detachment and reattachment groups. The proliferation of RPE in situ was detected by hybridization with probe of mRNAs of Ki-67 antigen and the signs of hybridization between the different regions and time after treatment were compared respectively. Results: @9 . Of 22 cases, 16 patients of open injury complicated RD in 14 cases (87.5%) 11 operated cases within 1.5 months were all open injury complicated RD with an averaging course of 22 2 days, and the Ki-67 labeling index (LI) was 38.27%. While 6 patients of closed injury complicated RD in 2 cases (33.33%), and Ki- 67 LI was 18% (P~0.003 1) with an averaging course of 4.2 months (p=O.004 1). The proliferation of migrated RPE increased promptly after the injury (55% at 33 days for Ki-67 LI, 50% at 54 days for PCNA LI), and reached the peak from 1 to 2 months The Ki-67 LI were 38.27%, and 10.82% for <1 5 and >1 5 months of groups respectively (P0.0000), The corresponding values for PCNA LI were 32.91%, and 18.09% respectively (Th=O.0273). The proliferating 5 activity would decrease as the prolongation of course and the development of PVR. The bPVR and cPVR showed 40.14% and 15.38% of Ki-67 LI respectively (P=0.00 13). The low level of LI appeared after 4 months. In addition, Ki-67 LI of RPE in open injury within 5 months after injury was much higher than that of closed one (35.5% and 2 1.6%, P0.0472). Similarly, Ki-67 LI of specimen obtained from the injured trace was much higher than that of specimen far away from the trace (35.08% and 12.5%, P 0.0241). ~. 74.96%, 25.69% and 2.68% of RPE in situ in detached group expressed mRNA of Ki-67 antigen for 6, 14 and 30 days respectively (P0.0000). The rates in reattached group were 43.03%, 11.7% and 2.69% respectively (P=0.0000), demonstrated lower expression than former group for 6 and 14 days (P0.0065 and P=0.0127). The RPE of areas nearby the detached retina lowly expressed mRNA after 6 days of treatment than that of the detached retina in two groups (P=0.0463, P=0 006). The statistical difference disappeared when compared the signs of 14 days. The RPE of near the detached retina in reattached group showed low expression than that in...
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