Effect Of Inducible Overexpression Of Bak And P27～(KIP1) On Apoptosis And Cell Cycle Regulation In The Hepatocellular Carcinoma Cells

Apoptosis and cell cycle regulation play an important role in tumor pathogenesis and therapy. To explore the exact mechanisms of apoptosis and cell cycle regulation will provide some new thought for overcoming tumors. Hepatocellular carcinoma (HCC) is one of high prevalent malignant neoplasm in China. A major research work in our laboratory has been focused oh it. Based on previous research findings, our current research work is intended to elucidate the effect of inducible overexpression of Bak, a proapoptotic member of Bcl-2 gene family, on apoptosis and cell cycle regulation in the hepatocellular carcinoma cells. We further investigate a cyclin dependent kinase inhibitor, p27^KIPI, which has been proved to exert its effect on the process mentioned above, on apoptosis and cell cycle regulation. The main research findings of this study are categorized as follows:1. We have developed a modified TUNEL assay for detection of apoptosis in situ on formalin-fixed, paraffin-embedded sections.2. Immunohistochemical analyses and in situ hybridization for Bax or Bak...