Experimental Studies On The Tumorigenic Mechanism And Gene Therapy Of Epstein-Barr Virus

Epstein—Barr Virus (EBV) is one kind of tumor virus. It is closely related to the development of Burkitt's lymphoma (BL) and undifferentiated nasopharyngeal carcinoma (UNPC). Recent reports state that EBV genomes have been detected in other neoplasms, including T -cell lymphomas,Hodgkin's disease, salivary carcinoma, thymus cancinoma and lung sarcirnoma. It is evident that EBV can infect numerous different cell types. Although it is clear that EBV is a special virus spread widely in human population, but to what kind of cancer and the precise role of EBV in the development of tumors is still uncertain. Although molecular biological and immunological evidence suggests that EBV is closely related to the development of NPC. In present literature, EBV as an etiological factor was not yet determined. Up to date, no direct evidence which shows EBV infection can induce carcinoma of nasopharyngeal epithelia cells. Therefore, we performed the following 4 experiment studies.1.Studies on the expression of EBERs in human nasopharyngeal carcinoma cell lines by in situ hybridization. Human nasopharyngeal carcinoma cell lines(CNE—1, CNE—2, CNE—3) were detected by in situ hybridization with Dig-labelled EBERs probe. Results showed that in both well and poorly differentiated NPC had EBV DNA presentation,which were richly located, specifically in the nuclei of cancer cells. Positive rate were >92%. The EBERs were detected as early as 36h after EBV infection and it is comparatively stable. In situ hybridization...