Generation Of Repertoires Of Human Antibodies Displayed On Phages From The Patients With HFRS And Identification Of Anti-HTV Antibody Fragments

Epidemic hemorrhagic fever (EHF)is caused by Hantavirus(HTV) , and EHF is named as hemorrhagic fever with renal syndrome (HFRS). The disease is an endemic and seasonal infectious disease that carries a great threat on human health and life. Therefore the research on the effective measures to prevent and cure EHF is a hard researching task faced to medical workers and researchers yet.The antibody against HTV should be placed on the most important list of the measures to prevent and cure EHF. Many researches have be done but there is no significant find in this field. The monoclonal antibody (McAb) from mouse or rat is of heterogenetic stimulation to human body. Nowadays, there is neither the effective neutralizing antibody nor real human genetic engineering antibody against HTV.Genetic engineering antibody (the third generation of antibody) may reduce or overcome the inadvantages of McAb from hybridoma (the second generation of antibody). The technology of repertoire of antibodies displayed on phage was reputed as a revolution in the third revolution of antibody's research. By it, the genetic engineering antibody may be made with bypassing immunization and hybridoma. Wilfully, any real human genetic engineering and interested antibody may be made by only using the methods of genetic engineering. The main criteria to judge an advanced research on some genetic engineering antibody may be :â‘  using the technology of repertoire of antibodies displayed on phage and bypassing immunization and hybridoma; â‘¡making antibody from human body; â‘¢getting a secreting expression after fusion expression.