| Objection: To study on the changes of rejection-related cytokines of renal transplantation in the rat liver, heart and brain, and explore the possible mechanisms between acute rejection reaction and damage of those organs, further to prevent from of the damage after renal transplantation.Methods: Retrospective analysed the complications of liver, heart and brain in 108 cases with renal transplantation, and compared with the 24 cases developed acute rejection. Meanwhile, a model of rat renal transplantation was established for further study on the mechanism of the rejection-related organ damage. 70 rats were divided into two groups, 10 rats into control group and 60 into experimental group(30 rats were donors, the others were recipients). After fasting 12 hours preoperative, rats were anaesthetized(injected 10% chloral hydrate 35mg per 100g by weight into rats peritoneal cavity) and renal transplantation were operated on. All rats were sacrificed 10 days post-operatively. The major investigating markers: 1. the histopathologic changes of the liver, heart and brain microscopically; 2. the expressions of the inflammatory cytokine factors as RANTES, INF-γ R, INF- γ , MMP-2, TIMP-2 and apoptosis-related factors as Fas, Fas-L, perforin, Granzyme B in the rats liver, heart and brain, immunohistochemically; 3. the expressions of IL-2mRNA, IL-6mRNA and INF- γ mRNA probed by hybridization in...
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