Cell Cycle Broken Types In Clinical Gastroenteric Tumor

Background and aims: It is widely believed that cancer is a kind of cell cycle disease. The relationship of cyclins and cancer has been noted for a long time. The primary cyclins (A, B1, D1, and E) are crucial for cell cycle progression. But gastroenteric tumor has been found the expression of cyclins (A, B1, D1, and E) was disaccord. We try to investgate the expression of human major cyclins in gastroenteric tumor, and discover the laws and roles of cyclins expressed in clinical tumor.Methods: In this study, we investigate the expression of cyclins (A, B1, D1, and E) in tumors. Flow cytometry (63cases) and Immunohistochemistry (88 cases) were used to detected the expression of human major cyclins in gastroenteric tumor.According to the result to classify and analyse.Results: In 151 specimens which were detected all most 52.5%(79caes), 51.0 %(77 cases),65.6%(99cases) and 56.3%(82cases)tumors' cyclins (A.B1.D1, and E) were overexpressed (p>0.05). And the result has no difference.Conclusions: The expression of human major cyclins in gastroenteric tumor was disaccord. Different cell cycle broken types were existed in clinic tumor. Background and aims: We have noted that there is different cell cycle broken typesexisted in clinic tumor. We based on the theory of cell cycle and tumorigenesis to detectthe expression of human major cyclins (A, B1, D1, and E) in gastroenteric tumor.According to the expression changes of 4 major cyclins, the different cancers will beclassified various cell cycle type at cellular molecular biology level, examined the cellcycle broken types with patients' clinical data and clinical tumor biological behavior.Methods: In this study, Flow cytometry and Immunohistochemistry were used to detectthe expression of human major cyclins in gastroenteric tumor. Based on the expressionchanges of 4 major cyclins, the different clinical cancers will be classified 4 cell cyclebroken types. The results were analysed with patient TNM stage, the rates of thedifferentiation type and lymph node.Results: The level of cyclins expressed in 151 gastroenteric tumors was declininggradually from type I to type IV.It was shown relationship between the rates of of lymphnode and poor differentiation. Clinic stage (TNM stage) increasing gradually fromtype I to type IV (p<0.05).Conclusions: In our studuy we have classified 4 cell cycle broken types in clinical tumor.The result has comfirmed the relationship between the laws of cyclins expressed andclinical tumor biological behavior. The cell cycle brokentype will be benefits to the early diagnosis and prognosis of cancer, even to the strategyselection in treatment.The cell-cycle type may be as an available classification to designnew treatment strategy to improve patients' survival. Objective: Detect the expression of CyclinA, B1 , D1 , E and the chemosensitivity of some cancer drugs in 45 gastroenteric tumor cases . To investigation the relationships between the cell cycle broken type and the chemosensitivity in clinical gastroenteric tumor.Methods: Immunohistochemistry were used to determine Cyclin A, B1, D1, E expression and in 45 gastroenteric cancer cases. Meanwhile detect the Chemosensitivity of sever chemotherapeutics in the same cases by MTT assay. Result: A significant difference of chemosensitivity was found in different cell cycle broken type.From broken type I to IV chemosensitivity was decreased. Conclusion: There is significant dependability between the cell cycle broken type and chemosensitivity of after operation, this can be used to guide how to choice the chemotherapeutics and combination chemotherapy after operation.