| Objective MTTof jiangzhitong (total flavonoids) and jiangzhiduotang (polysaccharides) of a Chinese medicinal formula were evaluated.The protective efects and the possible mechanism of jiangzhitong were explore on CC14-induced acute hepatic injury and steatohepatitis in rats.Methods:Experiment one: In vitro, observed MTT of jiangzhitong and jiangzhiduotang from a Chinese medicinal formula at a concentration range of 1~100μg·ml-1.Experiment two: Protective effects of jiangzhitong on CC14-induced acute hepatic injury in rats. seventy two male Wistar rats were randomized into six groups.Oral treatment with jiangzhitong at doses of 50 ,100 and 200mg·kg-1 body weight were administered once daily for a week.After the last administration, all groups except the normal control were received singe dose of CCl4 (2ml·kg-1 in peanut oil, i.p) to induce acute hepatic injury. Serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), the level of liver index (LI), and the contents of malondialdehyde (MDA), the activities of superoxide dismutase (SOD), reduced glutathione hormone (GSH), catalase (CAT), nitric oxide (NO), nitric oxide synthase (NOS) in liver tissue and hepatic pathological changes were also observed.Experiment three: The effects of jiangzhitong on CCl4-induced steatohepatitis in rats. 78 Wistar rats were divided into normal control group (n=12) and CCl4 treatment group (n=66) randomly according of body weight. Rats were treated with sublethal doses of carbon tetrachloride (40% CCl4 in peanut oil,3 ml·kg-1 body weight sc.once three day ) for 5 weeks to induce steatohepatitis. After the steatohepatitis model was established, the therapeutical experiment was executed, the rats with steatohepatitis were randomly divided into 5 groups .Groups of rats were treated with jiangzhitong (50, 100, 200 mg·kg-1 per day, respectively, p.o.) for 5 weeks. Control rats were treated with normal saline for 5 weeks. The following parameters were detected in all groups: the levels of body weight and liver index(LI), the activities of AST and ALT in serum; the content of albumin(ALB),total protein(TP) and NO, the activities of SOD, NOS, the contents of MDA, GSH, and the elevels of hydroxyproline(Hyp), free fatty acid(FFA) of liver tissues,the changes of histology.Results:Experiment one:The cytotoxicity of jiangzhitong and jiangzhiduotang toward HL-7702 hepatocytes was showed that concentrations of 1~100μg·ml-1 were not toxic to the cells growth.Experiment two: CCl4- induced hepatotoxicity in rats, as judged by the raised of serum enzymes, AST, ALT, was prevented by pretreatment with the jiangzhitong, demonstrating the hepatoprotective action. Antioxidant activity of jiangzhitong was also demonstrable by approach normal levels of MDA, SOD, CAT, and GSH in the liver homogenate. Moreover ,jiangzhitong treatment were decreased the production of the inflammatory mediator NO,NOS compared to the CCl4 control group.Experiment three: jiangzhitong ameliorated the increase in plasma ALT, AST, increased the ALB and the TP levels caused by repeated CCl4 intoxication in rats.Moreover, jiangzhitong significantly improved the CCl4-induced decrease of hepatic CAT and SOD activities. Jiangzhitong also restored the decrement in the glutathione content of hepatic tissues in CCl4-intoxicated rats. Furthermore, it also inhibited the formation of lipid peroxidative products and Hyp, FFA.Histopathological changes of hepatic lesions induced by carbon tetrachloride were significantly improved by treatment with jiangzhitong.Conclusion: The present investigation was to verify the hepatoprotective effect of jiangzhitong. The hepatoprotective action of jiangzhito is likely related to its potent antioxidative and anti-inflammatory activity. Neutralizing reactive oxygen species by nonenzymatic mechanisms and enhancing the activity of original natural hepatic-antioxidant enzymes may be the main mechanisms of jiangzhitong against CCl4-induced injury.
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